Journal: Genesis (New York, N.Y. : 2000)

Article Title: Cadherin-7 mediates proper neural crest cell-placodal neuron interactions during trigeminal ganglion assembly

doi: 10.1002/dvg.23264

Figure Lengend Snippet: Cadherin-7 protein localizes to HNK1-positive neural crest cells but not Pax3-positive placode cells or placodal neurons. Representative serial/adjacent transverse sections taken at the axial level of the forming ophthalmic lobe of the trigeminal ganglion at HH15 followed by triple-label immunohistochemistry for either Pax3 (a, green), HNK-1 (b, red), and Annexin A6 (c, purple), or Cadherin-7 (e, green), HNK-1 (f, red), and Annexin A6 (g, purple). (d′) Higher magnification image of the boxed region in (d) shows placode cells or placodal neurons (Pax-3- and Annexin A6-positive cells, arrowheads) that are not labeled with HNK-1 and thus are different from HNK-positive neural crest cells (arrows). (h′) Higher magnification image of the boxed region in (h) reveals Cadherin-7 immunoreactivity solely in HNK-1-positive neural crest cells (arrows), while Annexin A6-positive placodal neurons (arrowheads) are devoid of Cadherin-7. Rostral and caudal orientation and the ophthalmic lobe of the trigeminal (opV) are indicated in (d) and (h). DAPI (blue) labels cell nuclei. e = ectoderm. Scale bar in (a) is 67 μm and applicable to (b–h), while scale bar in (d′) is 20 μm and applicable to (h′)

Article Snippet: To further demonstrate localization of Cadherin-7 to neural crest cells, we performed additional immunohistochemistry experiments on collected adjacent serial sections (due to antibody incompatibility issues), examining Cadherin-7, HNK-1 (neural crest cells), and Annexin A6 (placodal neurons) on one set of sections, and Pax3 (a marker of opV placode cells in the surface ectoderm or placodal neurons in the mesenchyme; [ Stark, Sechrist, Bronner-Fraser, & Marcelle, 1997 ]), HNK-1, and Annexin A6 on the adjacent sections ( ).

Techniques: Immunohistochemistry, Labeling